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Inflammation in adipose tissue of SIRT1+/− mice on MFD. Epididymal fat was collected from mice at 28 wk of age and used in the analysis. A, H&E staining of epididymal fat. B, Lipogenic genes in epididymal fat. C, mRNA for adipokines and Pref-1. D, mRNA for inflammatory genes. E, mRNA for endothelial and angiogenic genes. F, Oil-Red O staining in differentiated MEFs. Values are the means ± se (n = 7). *, P ≤ 0.05. Apelin, an angiogenic factor produce by adipocytes.
