Table 1.
Association of liver and fat tissue weight with body weight
| Assay | MFD
|
HFD
|
||
|---|---|---|---|---|
| WT | SIRT1+/− | WT | SIRT1+/− | |
| Body weight (g) | 45.9 ± 1.1 | 47.2 ± 1.6a | 46.6 ± 1.9 | 49.0 ± 1.6a |
| Fat mass (g) | 14.9 ± 0.3 | 16.9 ± 0.4a | 18.5 ± 1.3 | 21.3 ± 0.5a |
| Lean mass (g) | 26.5 ± 0.9 | 26.0 ± 1.0 | 25.5 ± 0.3 | 25.7 ± 0.9 |
| Liver (g) | 1.75 ± 0.13 | 2.49 ± 0.15a | 1.64 ± 0.21 | 2.37 ± 0.28a |
| Liver/body weight ratio | 0.04 ± 0.003 | 0.05 ± 0.003a | 0.04 ± 0.005 | 0.05 ± 0.006a |
| Brown fat (g) | 0.20 ± 0.01 | 0.21 ± 0.01 | 0.21 ± 0.03 | 0.24 ± 0.03 |
| Epididymal fat (g) | 1.58 ± 0.15 | 1.37 ± 0.11 | 2.75 ± 0.09 | 2.45 ± 0.23 |
| Retroperitoneal fat (g) | 0.90 ± 0.04 | 1.05 ± 0.11 | 0.99 ± 0.10 | 1.10 ± 0.09 |
| Subcutaneous fat (g) | 12.42 ± 0.08 | 14.48 ± 0.28a | 14.76 ± 1.15 | 17.75 ± 0.53a |
| TAG (mg/dl) | 9.3 ± 0.5 | 8.6 ± 0.7 | 13.6 ± 2.2 | 10.7 ± 0.3a |
| Glycerol (mg/dl) | 7.2 ± 0.4 | 7.6 ± 1.2 | 8.7 ± 0.4 | 8.0 ± 0.7 |
| Cholesterol (mg/dl) | 134.9 ± 6.7 | 144.7 ± 10.0 | 263.4 ± 7.1 | 263.4 ± 11.3 |
Livers were collected from mice at 28 wk of age. In the MFD group, the mice were fed on the diet from 3 wk of age. In the HFD group, the mice were fed on LFD (regular chow) for 12 wk and then fed on HFD for 16 wk. Mice were euthanized using CO2. The sc fat is obtained from the total body fat by exclusion of epididymal and retroperitoneal fat. The total body fat contains fat in liver. Blood was collected first, and then liver, epididymal fat, retroperitoneal fat, and brown fat tissues were taken and weighed for the tissues to body weight ratio. Subcutaneous fat = (fat mass − epididymal fat − retroperitoneal fat). TAG, glycerol, and cholesterol were determined in plasma. Values are means ± se (n = 6 mice).
P ≤ 0.05.