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Gene expression measured by quantitative RT-PCR. Expression of Hes1, Vegfa, Vegfr1, and Iqgap1 was determined by quantitative RT-PCR using RNA extracted from the brains of E18.5 embryos collected from eight Meg3-null embryos and seven wild-type (WT) littermates of six litters. KO, Knockout. Values are the mean fold change of quantitative RT-PCR results relative to wild-type. ***, P ≤ 0.005; **, P ≤ 0.01; *, P ≤ 0.05.
