Table 1.

Primers used for quantitative RT-PCR

Gene name	Gene symbol	Forward primer	Reverse primer	Annealing temperature (C)
Delta-like 4	Dll4	GTTGCCCTTCAATTTCACCT	AGCCTTGGATGATGATTTGG	55
Glyceraldehyde-3-phosphate dehydrogenase	Gapdh	AGGTCGGTGTGAACGGATTTG	TGTAGACCATGTAGTTGAGGTCA	58
Hairy and enhancer of split 1	Hes1	CCAGCCAGTGTCAACACGA	AATGCCGGGAGCTATCTTTCT	58
IQ motif containing GTPase activating protein 1	Iqgap1	GATGTGACCCCTGAACAAGC	CTCTGCGTTCTCGTCACC	58
Neuropilin 1	Nrp1	CGTGGAAGTAATTGATGGGGAG	CATAGCGGATGGAAAACCCTG	56
Vascular endothelial growth factor A	Vegfa	CTTGTTCAGAGCGGAGAAAGC	ACATCTGCAAGTACGTTCGTT	57
Vascular endothelial growth factor receptor 1	Vegfr1	TGGACCCAGATGAAGTTCCC	GCGATTTGCCTAGTTTCAGTCT	57
Vascular endothelial growth factor receptor 2	Vegfr2	AGCACTGGTCCTATGGGTTG	GGTTCTGCCATTTGATCCA	55
Wiskott-Aldrich syndrome-like (human)	Wasl	GGAACTGTATGTGGGCAAAGAAGT	GACAAGTATCTCCAGCAAAGGTATG	58