Table 1.

Stereological enumeration of Sertoli and germ cell populations

Group	n	Sertoli cells	Spermatogonia	Early spermatocytes	Pachytene spermatocytes	Round spermatids	Elongated spermatids
hpg/WTa	6	1.60 ± 0.20	0.99 ± 0.11	0.81 ± 0.17	0.09 ± 0.04	0.05 ± 0.05	0.01 ± 0.01
hpg/WT + E2b	6	1.77 ± 0.18	1.99 ± 0.15	4.82 ± 0.57	1.03 ± 0.17	0.81 ± 0.19	0.09 ± 0.05
hpg/WT + DHTc	5	1.54 ± 0.12	1.74 ± 0.20	4.45 ± 0.24	1.51 ± 0.09	12.2 ± 0.52	11.2 ± 0.47
hpg/αERKO	5	1.39 ± 0.08	0.58 ± 0.07	0.53 ± 0.08	0.06 ± 0.02	0	0
hpg/αERKO + E2b	5	1.58 ± 0.08	0.62 ± 0.07	0.26 ± 0.08	0	0	0
hpg/αERKO + DHTc	5	1.45 ± 0.16	1.16 ± 0.10	3.18 ± 0.27	0.86 ± 0.18	6.20 ± 0.64	5.58 ± 1.02
hpg/βERKO	6	1.39 ± 0.08	1.20 ± 0.12	2.36 ± 0.42	0.36 ± 0.08	1.19 ± 0.44	0.45 ± 0.26
hpg/βERKO + E2b	6	1.79 ± 0.25	2.20 ± 0.34	4.81 ± 0.74	1.20 ± 0.20	4.68 ± 1.95	2.33 ± 1.28
hpg/βERKO + DHTc	5	1.47 ± 0.17	2.49 ± 0.30	5.27 ± 0.37	1.77 ± 0.53	14.5 ± 1.50	12.4 ± 1.47
P value
Genotyped		0.50	<0.001	<0.001	<0.001	<0.001	<0.001
Treatmente		0.13	<0.001	<0.001	<0.001	<0.001	<0.001
G × Tf		0.97	0.038	<0.001	0.133	0.002	<0.001

All data from study 2 expressed as mean ± sem and million cells per testis. 

^aWT means wild-type genetic background with respect to ERα and ERβ; 

^bE2 administered by 1 cm subdermal implants filled with recrystallized E2 diluted 1:1000 with cholesterol; 

^cDHT administered by 1 cm subdermal implant filled with crystalline DHT; 

^dGenotype (hpg/WT vs. hpg/αERKO vs. hpg/βERKO) main effect from two-way ANOVA; 

^eTreatment (untreated vs. E2 vs. DHT) main effect from two-way ANOVA; 

^fGenotype × treatment (G × T) interaction term from two-way ANOVA.