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. 2010 Apr 7;43(2):51–59. doi: 10.1267/ahc.09028

Fig. 3.

Fig. 3

Whole mount double immunofluorescence staining for c-Ret and cytokeratin at E12.5. Arrows indicate tips of the ureteric bud. (A, D, G) ICAT+/+ kidney. (B, E, H) ICAT−/− kidney. (C, F, I) ICAT+/+ kidney (negative control). F: Anti c-Ret antibody is replaced by the same concentration of normal rabbit IgG. I: Anti cytokeratin antibody is replaced by the same concentration of normal mouse IgG. (D, E) c-Ret. c-Ret is mainly localized in the branched ureteric bud tips. The intensity of the c-Ret immunoreactivities was identical in ICAT+/+ and ICAT−/−. (G, H) Cytokeratin. Cytokeratin is localized in the ureteric bud epithelium. In the ICAT+/+ kidney, the ureteric bud has six branched tips (G, arrows 1–6) while in the ICAT−/− kidney the ureteric bud has only two branched tips (H, arrows 7, 8). Dotted circles indicate the outline of the kidney. Asterisks (B, E, H) indicate Wolffian duct. Bar=200 µm.