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Immunohistochemistry of pituitary PRLoma in F344 rats. Staining of PRL (A to C), VEGF (D to F), Flk-1 (G to I). Counterstaining was done with hematoxylin. Bar=20 µm. J and K: Gene expression of VEGF (J) and Flk-1 (K) in pituitary PRLoma in F344 rats. The columns show the mean value of the relative expression of VEGF and Flk-1 compared with GAPDH as an endogenous control. *: P<0.05, E2 versus E2+G6-31 group.
