Skip to main content
. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Mucosal Immunol. 2010 Mar 10;3(3):291–300. doi: 10.1038/mi.2010.6

Fig. 1. RSV infection induces differentiation of AA-Mϕ.

Fig. 1

(A) Highly purified WT BALB/c BAL macrophage cultures were treated with medium only or infected with RSV. Supernatants were collected at the indicated time points and analyzed for IL-4 and IL-13 by ELISA. (B) WT BALB/c BAL macrophages were treated with medium alone, rIL-4, or RSV. Gene expression was analyzed by real-time PCR. Data are means ± SEM from a single representative experiment (N = 4). (C) WT C57BL/6 mice were mock- or RSV-infected and lungs harvested 4 d p.i. Frozen lung sections were stained for nuclei (DAPI; blue), F4/80 (green), and arginase-1 (red), then viewed by confocal microscopy. Arrows are positioned identically on each panel within a treatment to provide a reference point. The arrowhead in panels f–j illustrates a rare cell that is positive for F4/80, but not for arginase-1.

HHS Vulnerability Disclosure