Table 1.
Oligonucleotide primers for RT-PCR.
| Gene Detected | Primer | Sequence | Product Size | Location | Notes |
|---|---|---|---|---|---|
| TP63 TA (exons 1-4, no 3') | F | TGTATCCGCATGCAGGACT | 127 | exon 3 | unique to TA |
| R | CTGTGTTATAGGGACTGGTGGAC | exon 4 | |||
| TP63 ΔN (exons3'-4, no 1-3) | F | GAAAACAATGCCCAGACTCAA | 125 | exon 3' | unique to ΔN |
| R | TGCGCGTGGTCTGTGTT | exon 4 | |||
| TP63 α (exons 10-14, no 15) | F | TCAGTTTCTTAGCGAGGTTG | 118 | overlaps exon 12 and 13 | unique for α |
| R | ATTTTCAGACTTGCCAGATC | exon 14 | |||
| TP63 β (exons 10-12,14, no 13 or 15) | F | AGCATTGTCAGGATCTGG | 113 | overlaps exon 12 and 14 | unique for β |
| R | GAGATGAGAAGGGGAGGA | ||||
| TP63 γ (exons 10 & 15) | F | AAACATCTCCTTTCAGCC | 115 | overlaps exon 10 and 15 | unique for γ |
| R | GGTACACTGATCGGTTTG | exon 15 | |||
| TBP | F | CACGAACCACGGCACTGATT | 89 | ||
| R | TTTTCTTGCTGCCAGTCTGGAC |