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. Author manuscript; available in PMC: 2010 May 25.
Published in final edited form as: Cell Host Microbe. 2008 Oct 16;4(4):374–386. doi: 10.1016/j.chom.2008.08.016

Figure 3. Characterization of pDC-TLR response during LCMV infection.

Figure 3

Spleen pDCs (5 × 105 cells/ml) were FACS–purified as CD11c+B220+120G8highCD11b−CD3−NK1.1−CD19− from uninfected (n=3, white bars), ARM (n=10, striped bars) or Cl 13 (n=10, black bars) infected mice at day 5 pi. Cells were cultured with medium alone (none) or CpG-1668 during 15 h. A) IFN-I activity was measured in the supernatant by luciferase bioassay, other cytokines and chemokines indicated were determined by multi-plex ELISA. (B and C) CPG-stimulated pDCs were stained with anti-IAb and anti B7-2 mAbs and analyzed by FACS. B) Histrograms depict expression of the indicated molecules. Thin line, unstained control. C) Mean fluorescence intensity (MFI) for each molecule is shown.