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. Author manuscript; available in PMC: 2011 Apr 13.
Published in final edited form as: Biochemistry. 2010 Apr 13;49(14):3049–3059. doi: 10.1021/bi901953j

FIGURE 6.

FIGURE 6

Vesamicol saturation curves for E309K at pH 7.4 (◆), pH 8.5 (○), pH 9.3 (◇), and pH 10.2 (□). The concentration of [3H]vesamicol along the x-axis includes protonated and unprotonated forms of vesamicol. Eq. 2 and 3 were fitted simultaneously to the data as described in Results. Table 4 lists the derived parameter values.