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. Author manuscript; available in PMC: 2011 Apr 13.
Published in final edited form as: Biochemistry. 2010 Apr 13;49(14):3049–3059. doi: 10.1021/bi901953j

Table 2. Vesamicol binding to E309 mutants at pH 7.4a.

WT
or Mutant		 Kv (nM) Bmax
(pmol/mg)
WT 18 ± 3 63 ± 2
E309Q 25 ± 6 12 ± 1
E309D 18 ± 8 18 ± 2
E309A 81 ± 14 9.8 ± 0.7
E309K 49 ± 8 36 ± 2
D398N nb b
E309Q-D398N nb b
E309D-D398E nb b
a

A hyperbola was fitted independently to each set of data shown in Fig. S1. A value in boldface is significantly different from that of wild-type.

b

No binding.