Table 5. Equilibrium binding of ACh to E309 mutants at pH 7.4a.
| WT or Mutant |
KAChHA, KAChLA (mM) |
FHA |
|---|---|---|
| WT | 6.6 ± 1.5, 190 ± 120 |
0.75 ± 0.08 |
| E309Q | 11 ± 1, ndb |
0.91 ± 0.14 |
| E309D | 24 ± 6, ndb |
0.73 ± 0.25 |
| E309A | 34 ± 4 | 1 |
| E309K | 140 ± 10 | 1 |
| D398N | nvbc | |
| E309Q-D398N | nvbc | |
| E309D-D398E | nvbc | |
a
Data are shown in Fig. S2. A value in boldface is significantly different from that of wild-type.
b
Not determined because the regression error was too large.
c
No [3H]vesamicol binding required to do the measurement.