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. Author manuscript; available in PMC: 2011 Apr 13.
Published in final edited form as: Biochemistry. 2010 Apr 13;49(14):3049–3059. doi: 10.1021/bi901953j

Table 6. Transport of ACha.

WT
or Mutant		 KM (mM) Turnover (min−1)b pK3 pK4
WT 0.75 ± 0.3 7.5 ± 0.8 5.9 ± 0.1 8.4 ± 0.1
E309Q 1.2 ± 0.5 9.1 ± 1.5 6.1 ± 0.1 8.6 ± 0.1
E309D 0.75 ± 0.4 5.1 ± 1.0 6.2 ± 0.2 7.4 ± 0.2
E309A nt c
E309K nt c
E309D-D398E 0.9 ± 1.3 2.5 ± 1.2 6.0 ± 0.2 8.2 ± 0.2
D398N nt c
E309Q-D398N nt c
a

Saturation data for transport and regression fits are in Fig. S3, and pH-transport data and regression fits are in Fig. S4. A value in boldface is significantly different from that of wild-type.

b

Maximal transport rate per VAChT molecule in min−1. It is Vmax/Bmax, where Vmax is the Michaelis-Menten rate parameter.

c

No transport, so no parameter values are available.