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. 2010 Jan 12;16(4):1235–1237. doi: 10.1089/ten.tea.2009.0493

FIG. 2.

FIG. 2.

Shh treatment of cocultures (A) and pOB monocultures (B) results in an upregulation of proangiogenic factors at the mRNA level. (A) Mean values of relative gene expression of the proangiogenic factors VEGF, Angiopoietin1 (ANGPT1), and Angiopoietin2 (ANGPT2), the Shh receptor Ptch1 and the endothelial markers CD31 and in Shh-treated and untreated cocultures for 24 h (n = 6) and 14 days (n = 3). (B) Mean values of relative gene expression of VEGF, Angiopoietin1, Angiopoietin2, and Ptch1 in Shh-treated and untreated pOB monocultures for 24 h (n = 6) and 14 days (n = 3). RPL13A was taken as endogenous standard in both experiments (A, B). Gene expression of Shh-stimulated and nonstimulated cocultures and monocultures was compared by setting control cultures to 1 (reference value). Ptch1, Patched1; RPL13A, ribosomal protein 13A; VEGF, vascular endothelial growth factor; vWF, von Willebrand factor.