FIG. 6.

Shh treatment of cocultures (A) and pOB monocultures (B) results in an upregulation of osteogenic factors at the mRNA level. (A) Mean values of relative gene expression of different genes involved in osteogenesis in Shh-treated and untreated cocultures for 24 h (n = 6) and 14 days (n = 3). (B) Mean values of relative gene expression of different genes involved in osteogenesis in Shh-treated and untreated pOB monocultures for 24 h (n = 6) and 14 days (n = 3). RPL13A was taken as endogenous standard in both experiments (A, B). Gene expression of Shh-stimulated and nonstimulated co- and monocultures was compared by setting control cultures to 1 (reference value).