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. 2010 Jan 12;16(4):1235–1237. doi: 10.1089/ten.tea.2009.0493

FIG. 7.

FIG. 7.

Effect of Shh on osteoblastic differentiation in the coculture system consisting of pOBs and OECs. (A) Quantification of calcification in Shh-treated cocultures and pOB monocultures after 24 h (n = 6) and 14 days (n = 3) of Shh stimulation compared with unstimulated cultures evaluated by quantitative analyses of alizarin red staining. Values represent averages and alizarin red concentration is defined as μM alizarin red/mg protein. (B, C) ALP activity within the cell lysate in Shh-treated cocultures and pOB monocultures compared with untreated cultures after 24 h (n = 3) and 14 days (n = 3) of stimulation. Results are represented in averages. The standardized ALP activity is defined as mM pNP/mg protein and is depicted in (C). (B) The results in percentage to control = 100%. ALP, alkaline phosphatase.