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. Author manuscript; available in PMC: 2010 Sep 17.
Published in final edited form as: Sci Transl Med. 2010 Mar 17;2(23):23ra19. doi: 10.1126/scitranslmed.3000678

Fig. 1.

Fig. 1

Slit2N stabilizes the endothelium in vitro by enhancing VE-cadherin localization at the cell surface. (A) In vitro permeability was measured in HMVEC-lung cells stimulated with LPS, TNF-α, or IL-1β in the presence of Mock (see Materials and Methods) or Slit2N. (B) Robo4 or control siRNA knockdown-treated HMVEC-lung cells were stimulated with IL-1β in the presence of Mock or Slit2N to assess permeability in vitro. (C to E) HMVEC-lung cells were treated with Mock or Slit2N and subjected to membrane fractionation and subsequent immunoblotting for VE-cadherin (C), p120-catenin (D), or β-catenin (E). (F) HMVEC-lung cells were stimulated with Mock or Slit2N and subjected to immunofluorescence for VE-cadherin (green). White arrows, areas of enhanced VE-cadherin cell surface localization. For all experiments, n ≥ 3, and error bars represent SEM. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001.