Table 2.
Accumulated analysis of variation (unbalanced design therefore fitted through GenStat regression) for fresh weight and dry weight of seeds of Trifolium ambiguum harvested between 33 and 64 DAP (expt 1) or between 36 and 63 DAP (expt 2).
| Experiment 1 (pollinated 8 December 2004) |
Experiment 2 (pollinated 11 January 2006) |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fresh weight (mg) |
Dry weight (mg) |
Fresh weight (mg) |
Dry weight (mg) |
|||||||||||||
| Change | d.f. | SS | MS | VR | d.f. | SS | MS | VR | d.f. | SS | MS | VR | d.f. | SS | MS | VR |
| + DAP | 9 | 118·80 | 3·20 | 200·46*** | 7 | 3·35 | 0·48 | 24·28*** | 7 | 86·82 | 12·40 | 65·06*** | 7 | 0·26 | 0·04 | 1·34 |
| + Mat. | 1 | 0·34 | 0·34 | 5·13* | 1 | 0·31 | 0·31 | 15·65*** | 1 | 0·06 | 0·06 | 0·29 | 1 | 1·18 | 1·18 | 41·88*** |
| + No. | 1 | 2·23 | 2·23 | 33·81*** | 1 | 0·46 | 0·46 | 23·13*** | 1 | 1·92 | 1·92 | 10·07** | 1 | 0·59 | 0·59 | 21·11*** |
| + PosI | 2 | 1·74 | 0·87 | 13·20*** | 2 | 0·05 | 0·02 | 1·16 | 2 | 6·64 | 3·32 | 17·42*** | 2 | 0·20 | 0·10 | 3·48* |
| + DAP × Mat. | 5 | 0·84 | 0·17 | 2·54* | 4 | 0·68 | 0·17 | 8·63*** | 7 | 5·51 | 0·79 | 4·13*** | 7 | 0·47 | 0·07 | 2·39* |
| + DAP × No. | 8 | 0·93 | 0·12 | 1·76 | 7 | 0·38 | 0·05 | 2·78* | 7 | 1·42 | 0·20 | 1·07 | 7 | 0·26 | 0·04 | 1·32 |
| + Mat. × No. | 1 | 0·06 | 0·06 | 0·98 | 1 | 0·00 | 0·00 | 0·18 | 1 | 0·01 | 0·01 | 0·08 | 1 | 0·05 | 0·05 | 1·95 |
| + DAP × PosI | 14 | 3·48 | 0·25 | 3·77*** | 12 | 0·82 | 0·07 | 3·48*** | 13 | 3·68 | 0·28 | 1·48 | 13 | 0·42 | 0·03 | 1·16 |
| + Mat. × PosI | 2 | 0·25 | 0·13 | 1·93 | 2 | 0·01 | 0·00 | 0·16 | 2 | 0·63 | 0·32 | 1·66 | 2 | 0·11 | 0·06 | 1·98 |
| + No. × PosI | 2 | 0·10 | 0·05 | 0·73 | 2 | 0·14 | 0·07 | 3·61* | 2 | 0·20 | 0·10 | 0·53 | 2 | 0·02 | 0·01 | 0·37 |
| + DAP × Mat. × No. | 3 | 0·05 | 0·02 | 0·25 | 2 | 0·04 | 0·02 | 1·04 | 6 | 0·57 | 0·09 | 0·49 | 6 | 0·36 | 0·06 | 2·12 |
| + DAP × Mat. × PosI | 3 | 0·30 | 0·10 | 1·53 | 2 | 0·06 | 0·03 | 1·44 | 7 | 1·65 | 0·24 | 1·24 | 7 | 0·12 | 0·02 | 0·60 |
| + DAP × No. × PosI | 3 | 1·01 | 0·34 | 5·14** | 2 | 0·47 | 0·24 | 11·92*** | 7 | 0·62 | 0·09 | 0·46 | 7 | 0·13 | 0·02 | 0·67 |
| + Mat. × No. × PosI | 1 | 0·31 | 0·31 | 4·78* | 1 | 0·18 | 0·18 | 9·36** | 1 | 1·15 | 1·15 | 6·03 | 1 | 0·00 | 0·00 | 0·13 |
| + DAP × Mat. × No. × PosI | 1 | 0·02 | 0·02 | 0·35 | 0 | 0·00 | 0·00 | 0 | 0 | 0 | 0 | 0 | ||||
| Residual | 128 | 8·43 | 0·07 | 105 | 2·07 | 0·02 | 92 | 17·54 | 0·19 | 92 | 2·59 | 0·03 | ||||
| Total | 184 | 138·89 | 0·75 | 151 | 9·03 | 0·06 | 156 | 128·42 | 0·82 | 156 | 6·77 | 0·04 | ||||
DAP, days after pollination; Mat., maternity (M1 or M2); No., seed number; PosI, position in inflorescence (top, middle, or bottom).
*P < 0·05; **P < 0·01; ***P < 0·001.
Position in the pod was not always recorded for single-seeded pods and therefore this factor was not included in the analysis. Changing the order of factors did alter the significance of some second-order interaction terms, but not of main effects or first-order interactions.