Table 2.
Membrane and compartment assignments for identified proteins.
| Category | Compartment | Flybase annotation | Percent integral1 | Number of proteins in category | Percentage of all proteins |
|---|---|---|---|---|---|
| Plasma Membrane | Plasma membrane | Plasma membrane | 60% (57/95) | 95 | 22% |
| Candidate Plasma Membrane Residents (Integral) | Not assigned | Membrane | 50% (10/21) | 74 | 17% |
| Integral to membrane | 100% (21/21) | ||||
| None but we predict ≥ 1 transmembrane domain | 100% (43/43) | ||||
| Candidate Plasma Membrane Residents (Peripheral) | Not assigned | Membrane | N/A2 11 proteins | 74 | 17% |
| None with no predicted transmembrane domains | N/A2 63 proteins | ||||
| Lipid particles | Lipid particles | Lipid particles | 22% (16/74) | 74 | 17% |
| Other | Cytoplasm and Nucleus | Cytoplasm Cytoskeleton Nucleus Ribosomes | 6% (4/69) | 69 | 16% |
| Mitochondrial | Mitochondrial | Mitochondrial | 18% (5/38) | 38 | 9% |
| Endoplasmic reticulum and Golgi | Endoplasmic reticulum and Golgi | Endoplasmic reticulum and Golgi | 38% (3/8) | 8 | 2% |
| All compartments | 37% (159/432) | 432 | 100% | ||
1Proteins predicted by hydropathy analysis to have ≥ 1 transmembrane domain
2Not Applicable - neither category has any predicted integral membrane proteins.
The table shows the number of proteins assigned to compartments based on their Flybase annotation in conjunction with our hydropathy analysis. The biggest single category has been annotated in Flybase as 'Plasma membrane'. The next two categories we predict will also contain a substantial fraction, which are in the PM compartment; however, this will obviously require experimental verification by interested investigators (see text for discussion).