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. 2010 Apr 29;6:24. doi: 10.1186/1744-8069-6-24

Figure 4.

Figure 4

The P1308L and V1298F mutations have different effects on repriming. Cells were held at -100 mV, and fast-inactivation was initiated by a 20-ms depolarization to 0 mV, followed by a recovery period (2-300 ms) at a recovery potential. The available channels were then measured with a 10-ms test pulse at 0 mV. Recovery fraction was calculated by normalizing the peak currents in response to test pulses, to peak currents of prepulses after various recovery durations (2-300 ms) at different recovery potentials, and plotted as a function of recovery potentials. A, B, and C, V1298F mutant channels show higher recovery fractions than WT channels at recovery potentials from -90 to -70 mV, whereas P1308L channels do not affect recovery fraction. D, Repriming kinetics was calculated by mono-exponential fits of the recovery rate at different recovery duration. V1298F channels showed faster repriming kinetics than WT channels at all testing potentials, whereas P1308L had no effect on the repriming kinetics.