TABLE 1.

Estimates of [ADO]_isf in isolated guinea-pig hearts

Method	Conditions	[ADO]v nM	[ADO]isf nM	ISF/Venous Ratio	Predicted [ADO]isf, nM	Reference
Transudate	Rest	30	180	6	73	Decking (1988)
Transudate	Rest	37	191	5	113	Mohrman (1990)
	Dipyridamole	90	785	9	623
SAH	Rest	9	80‡	9	13	Deussen (1988)
Epicardial disk	Rest	4	280	70	6	Tietjan (1990)
	Dipyridamole	27	1,190	44	69
Epicardial disk	Rest	17	154	9	35	Headrick (1991)
	Norepinephrine	461	496	1	1,071
Capillary transport	Rest	4	5–12	1.5–4		Wangler (1989)
	Dipyridamole*	44	191	4
Capillary transport	Rest	2	3–7	1.5–3.5		Gorman (1991)
	Norepinephrine†	45	166–324	4–7

Predicted [ADO]_isf was calculated from the cited study’s flow and [ADO]_v using average capillary transport parameters from Gorman et al., 1991. ISF, interstitial fluid; SAH, accumulation of S-adenosylhomocysteine. During dipyridamole it was assumed that endothelial adenosine uptake is blocked (PS_ecl = PS_eca = 0).

^*Constant pressure conditions.

^†Values after 20 min NE infusion.

^‡This value is for the cytosol of the myocyte, not ISF. (Reproduced from Gorman et al., 1991, with permission.)