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. Author manuscript; available in PMC: 2011 Jul 15.

Published in final edited form as: Anal Biochem. 2010 Mar 31;402(2):146–150. doi: 10.1016/j.ab.2010.03.036

A: Conjugation dependent changes in the functional (○) and unlabeled fractions (■). The unlabeled fraction was calculated using Poisson statistics and the functional fraction is the global linking vector, β.

B: The difference between the functional and unlabeled fractions has a maximum at a Fluorophore:Protein ratio of ~3 representing the optimum conjugation level. The dotted lines represent the +/- 10% range about the optimum and corresponds to a F:P ratio of ~ 2 – 5.

A: Conjugation dependent changes in the functional (○) and unlabeled fractions (■). The unlabeled fraction was calculated using Poisson statistics and the functional fraction is the global linking vector, β.

B: The difference between the functional and unlabeled fractions has a maximum at a Fluorophore:Protein ratio of ~3 representing the optimum conjugation level. The dotted lines represent the +/- 10% range about the optimum and corresponds to a F:P ratio of ~ 2 – 5.