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. Author manuscript; available in PMC: 2011 Jun 1.

Published in final edited form as: Biochemistry. 2010 Jun 1;49(21):4543–4553. doi: 10.1021/bi100061v

Multiple Dda molecules increase the amplitude of ssDNA formed, but do not change the kinetic step size, or the rate for unwinding. A. Re-plot of product amplitudes as a function of dsDNA length for the 12T (), 14T (), 21T (), and 28T () substrate sets. B. Re-plot of the unwinding rate constant () and the kinetic step-size () as a function of the estimated number of Dda molecules bound to the different substrates at the start of the reaction. One, two, three, or four Dda molecules were bound to substrate 12T, 14T, 21T, and 28T, respectively. Error bars are the errors in the fits to the data.

Inline graphic — Multiple Dda molecules increase the amplitude of ssDNA formed, but do not change the kinetic step size, or the rate for unwinding. A. Re-plot of product amplitudes as a function of dsDNA length for the 12T (), 14T (), 21T (), and 28T () substrate sets. B. Re-plot of the unwinding rate constant () and the kinetic step-size () as a function of the estimated number of Dda molecules bound to the different substrates at the start of the reaction. One, two, three, or four Dda molecules were bound to substrate 12T, 14T, 21T, and 28T, respectively. Error bars are the errors in the fits to the data.