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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biochemistry. 2010 Jun 1;49(21):4543–4553. doi: 10.1021/bi100061v

Table 2. Kinetic parameters determined from NLLS analysis of data in Fig. 1 for the substrate containing a 12 nt overhang (one molecule of Dda per substrate).

ku, s−1 step-size (m), bp unwinding rate (mku), bp s−1 Na
107 ± 12 2.4 ± 0.7 256 ± 74 19 bp
Individual kd values (s−1) for different lengths of dsDNA
12T16bp 12T20bp 12T24bp 12T28bp
6.0 ± 0.5 17.0 ± 1.0 16.2 ± 1.1 19.4 ± 2.6
a

N (the number of bp unwound per binding event) was estimated according to Eq. 3 by using the step-size, m, the ku value and the average of the kd values (21).