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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biochemistry. 2010 Jun 1;49(21):4543–4553. doi: 10.1021/bi100061v

Table 4. Kinetic parameters determined from NLLS analysis of data in Fig. 4A for the substrate containing a 14 nt overhang (two molecules of Dda per substrate).

ku, s−1 step-size (m), bp unwinding rate (mku), bp s−1 Na (bp per binding event)
66±7 3.1±0.9 206 ± 58 18 bp
Individual kd values (s−1) for different lengths of dsDNA :
14T16bp 14T20bp 14T24bp 14T28bp
6.9 ± 0.4 13.4 ± 0.7 10.5 ± 0.6 17.7 ± 1.8
a

N (the number of bp unwound per binding event) was estimated according to Eq. 3 by using the step-size, m, the ku value and the average of the kd values (21).