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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biochemistry. 2010 Jun 1;49(21):4543–4553. doi: 10.1021/bi100061v

Table 6. Kinetic parameters determined from NLLS analysis of data from Fig. 4C for the substrate containing a 28 nt overhang (four molecules of Dda per substrate).

ku, s−1 kc, s−1 step-size (m), bp unwinding rate (mku), bp s−1 Na (bp per binding event)
72±9 34±13 3.4 ± 0.7 248 ± 48 64 bp
Individual kd values (s−1) for different lengths of dsDNA
28T16bp 28T20bp 28T24bp 28T28bp
4.8 ± 0.5 3.6 ± 0.5 4.1 ± 0.4 3.2 ± 0.3
a

N (the number of bp unwound per binding event) was estimated according to Eq. 3 by using the step-size, m, the ku value and the average of the kd values (21).