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. Author manuscript; available in PMC: 2010 May 26.
Published in final edited form as: FASEB J. 2005 May 18;19(10):1371–1373. doi: 10.1096/fj.04-2770fje

Figure 2. Phenotype of cloned stromal cells.

Figure 2

A) Proteoglycans from culture media of 3 selected stromal clones (CPD 22) were assayed for keratan sulfate-containing proteoglycan using monoclonal antibody J19 with (+) or without (−) pretreatment with keratanase. B) mRNA pools for keratocan (shaded) and ALDH (open) were determined by quantitative RT-PCR in primary keratocytes, clonal stromal cells (CPD 24), and passage-four fibroblasts. Values are normalized to keratocytes = 100. C) Primary keratocytes were immunostained for f-actin (green) and vinculin (red). D) Clonal stromal cells (CDP 50) were stained similarly to C. E) Fibroblasts, passage four, were stained similarly to C and D.