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Marker expression during expansion of progenitor cells. RNA was purified from primary keratocytes (CPD 0) and from clonal progenitor cells at early passage (27 CPD) and late passage (60 CPD). Cells were maintained in cloning medium (CM) (open bars) or after a week in differentiation medium (solid bars). Relative mRNA abundance was calculated as a percentage of that of primary keratocytes. A) ALDH mRNA. B) keratocan mRNA.
