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. Author manuscript; available in PMC: 2010 May 26.
Published in final edited form as: FASEB J. 2005 May 18;19(10):1371–1373. doi: 10.1096/fj.04-2770fje

Figure 7.

Figure 7

PAX6 expression by stromal progenitor cells. A) PAX6 protein in cloned progenitor cells was immunostained (green) and counterstained for cytoplasmic myosin (red). B) PAX6 protein was not detected in primary keratocytes cultured under identical conditions. C) PAX6 was detected only in rare cells in intact corneal stroma (arrowhead). Keratocyte cell membranes were counterstained with DiD (red). D) Freshly isolated stromal cells were fixed and stained for PAX6 protein and then analyzed by flow cytometry. Solid line shows results with a PAX6 primary antibody. Dashed line shows a nonimmune rabbit IgG primary. Fluorescence intensity is shown as a logarithmic scale. Arrow indicates a population of stained cells representing 4% of the total. White bars = 50 μm.