Table 1.
Primers for stem cell gene RT-PCR
| Gene | Source* | Forward | Reverse |
|---|---|---|---|
| ABCG2 | NM_174222 | AACTTCTGCCCAGGACTCAA | ATGCCAAGGCTACGTGATTC |
| CD166 | NM_174238 | TCTCATGTTTGGCAAATGGA | GAAATGGGGCTTTGCTTACA |
| CD73 | NM_174129 | CTGAGACACCCGGATGAGAT | ACTGGACCAGGTCAAAGGTG |
| CD90 | TC288067 | ATACCCCTCCATCCTTCCAC | CACCTCTGCCAATACCACCT |
| Fhl1 | TC262429 | TGGCACAAAGACTGCTTCAC | TCTTGCATCCAGCACACTTC |
| SCF | AB033716 | GGGATCTGCAGTAACCGTGT | TTCCACCATCTCGCTTATCC |
| Six2 | TC297269 | CTCTCTTCCTTTGCCCTCCT | CCGAGAAACACTGAGGGGTA |
| Six3 | BF654243 | ACCATCAACAACTCCCAACC | AGCGGTGCTTGTCCTAGAAA |
| Bmi1/Flvi-2 | TC279460 | CTCCACCTCTTCCTGTTTGC | CCAGATGAAGTTGCTGACGA |
| Notch1 | TC263497 | GATGTGGTTCATGCATTTCG | CACACCTCCGACTTCTCACA |
| PAX6 | U73621 | TTTTCCTCCCTCCCTAACTCA | GATGCGTCAAAACGAAGTCA |
*
Source numbers starting with “TC” were obtained from The Institute for Genome Research Cattle Gene Index (www.tigr.org). Others are GenBank accession numbers.