FIG. 1.

Schematic of S-layer stabilized solid supported lipid membranes. In (a), the S-layer is directly recrystallized on the gold-coated surface. Subsequently, a lipid membrane can be formed, e.g., by the Langmuir-Schaefer technique. In (b), thiol-functionalized secondary cell wall polymers (SCWPs) are directly bound to the gold surface. On this biomimetic surface, S-layer proteins can be recrystallized and finally, a lipid membrane can be formed. In (c), the S-layer is directly recrystallized on the gold-coated surface. To take advantage of the S-layer protein, SCWP interaction, lipid-SCWP constructs are added to the membrane-forming lipid resulting in membranes anchored on the S-layer lattice and tethered by SCWP. As an option, on all three composite architectures, S-layer proteins can be recrystallized as a protecting coat with molecular sieve function.