Figure 3.
Dose-dependent inhibition of BoNT/A by 0831–1035 in a primary neuronal cell culture. Varying concentrations of 0831–1035 were added to the cell culture medium, followed by the addition of 0.5 nM BoNT/A. After 3 hr incubation, cell samples were subjected to immunoblot analysis. The degree of SNAP-25 cleavage was determined via scanning densitometry.