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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 1;878(19):1590–1598. doi: 10.1016/j.jchromb.2010.04.019

Figure 2.

Figure 2

Breakthrough curves for acetohexamide on an immobilized HSA column at applied concentrations (from left to right) of 10, 7.5, 5, 2.5, and 1 µM. Alternative detection wavelengths were used for some of the higher concentrations of analyte solutions to maintain a linear response in absorbance versus concentration during these studies, as described in the Experimental Section. Other conditions are given in the text.