FIG. 6.

D2S inhibits IL-6 (A) and IL-8 (B) release from neutrophils treated with LPS (0.05 μg/ml) from E. coli (black column) or purified LTA (10 μg/ml) from S. aureus (gray column). White columns represent values obtained in control (nontreated samples or neutrophils treated with indicated D2S amount). Error bars represent standard deviations from three measurements performed in duplicate. *, significantly different from neutrophil samples treated with LPS or LTA. Morphology of human neutrophils (C) in resting stage (C1) or with addition of yeast indicated by arrows (C2) in the presence of 10 μM (C3) and 20 μM (C4) D2S. Viability of neutrophils (D) after 1 h of incubation of their suspension with dexamethasone (▴), spermine (▵), and D2S (□). Error bars represent standard deviations from three measurements.