FIG. 3.

Effect of treatment duration and inhibitor concentration on replicon RNA level. Replicon cells were treated with various concentrations of SCH 900518, as indicated. The replicon cells were refreshed with the compound every 2 to 3 days, and the cells were split when they became confluent. The log RNA reduction at day 0 is set equal to 0. The model used to fit the curve is one-phase exponential decay. The reduction in the amount of replicon RNA is calculated as follows: dC_T = NS5B C_T − GAPDH C_T, where ddC_T = dC_T of treated samples − dC_T for the vehicle control and where the log RNA reduction = log(1/2^ddCT). IC₉₀, 90% inhibitory concentration.