TABLE 1.
Testing of virus susceptibility to favipiravir in plaque reduction assay with MDCK cellsb
| Strain designation | Subtype | Adamantane phenotype | M2 mutation | Oseltamivir phenotype | Zanamivir phenotype | NA mutationa | Favipiravir EC50 ± SD (μM) |
|---|---|---|---|---|---|---|---|
| A/Georgia/17/2006 | H1N1 | S | S | S | 1.46 ± 0.25 | ||
| A/Georgia/20/2006 | H1N1 | S | R | S | H274Y | 2.55 ± 0.06 | |
| A/California/27/2007 | H1N1 | S | S | S | 3.63 ± 0.06 | ||
| A/New Jersey/15/2007 | H1N1 | S | R | S | H274Y | 4.90 ± 0.06 | |
| A/Ecuador/5179/2008 | H1N1 | S | S | S | 2.48 ± 0.12 | ||
| A/Santiago/5248/2008 | H1N1 | S | R | R | D198E | 4.70 ± 0.06 | |
| A/Brazil/1067/2008 | H1N1 | S | S | S | 1.85 ± 0.06 | ||
| A/Brazil/1633/2008 | H1N1 | S | R | R | Q136K | 0.83 ± 0.06 | |
| A/Luhansk/18/2008 | H1N1 | R | G34E | R | S | H274Y | 2.93 ± 0.06 |
| A/New York/34/2008 | H1N1 | S | L26I | S | S | 0.19 ± 0.06 | |
| A/Washington/10/2008 | H1N1 | R | S31N | S | S | 3.25 ± 0.19 | |
| A/Florida/21/2008 | H1N1 | S | R | S | H274Y | 1.59 ± 0.06 | |
| A/Wisconsin/16/2008 | H1N1 | S | R | S | H274Y | 2.87 ± 0.06 | |
| A/North Carolina/02/2009 | H1N1 | R | S31N | S | S | 5.03 ± 0.06 | |
| A/Idaho/01/2009 | H1N1 | S | R | S | H274Y | 2.93 ± 0.06 | |
| A/Wuhan/395/1995-like | H3N2 | S | S | S | 5.99 ± 0.06 | ||
| A/Wuhan/395/1995-like | H3N2 | S | R | S | E119V | 5.41 ± 0.12 | |
| A/Bethesda/956/2006 | H3N2 | R | S31N | R | R | R292K | 1.21 ± 0.06 |
| A/Washington/01/2007 | H3N2 | R | S31N | S | S | 4.46 ± 0.06 | |
| A/Texas/12/2007 (clone) | H3N2 | R | S31N | R | S | E119I | 3.95 ± 0.19 |
| A/Texas/12/2007 (clone) | H3N2 | R | S31N | R | S | E119V | 5.22 ± 0.06 |
| A/Florida/01/2009 | H3N2 | R | S31N | S | S | 0.45 ± 0.06 | |
| A/New Hampshire/01/2009 | H3N2 | R | S31N | S | S | 4.08 ± 0.06 | |
| A/Massachusetts/03/2009 | H3N2 | R | S31N | S | S | 4.20 ± 0.06 | |
| B/Memphis/20/1996 | R | N/A | S | S | 1.21 ± 0.06 | ||
| B/Memphis/20/1996 | R | N/A | R | R | R152K | 0.57 ± 0.06 | |
| B/Rochester/01/2001 | R | N/A | S | S | 1.40 ± 0.06 | ||
| B/Rochester/01/2001 | R | N/A | R | S | D198N | 1.72 ± 0.06 | |
| B/New York/22/2008 | R | N/A | S | S | 5.30 ± 0.06 | ||
| B/Illinois/03/2008 | R | N/A | R | R | E119A | 3.00 ± 0.19 | |
| B/Illinois/47/2005 | R | N/A | S | S | 4.01 ± 0.06 | ||
| B/Michigan/20/2005 | R | N/A | R | S | H274Y | 5.03 ± 0.06 |
a
N2 amino acid numbering.
b
S, sensitive; R, resistant, based on surveillance criteria (50). Values reported represent means of experiments performed in duplicate on three separate occasions ± standard deviations. Boldface type, established resistance-conferring mutation.