FIG. 2.
Effect of the M230L mutation on viral replicative capacity. Virus stocks were prepared through transfection of HEK 293T cells with proviral clones pNL4-3WT and pNL4-3M230L, normalized for RT activity, and used to infect TZM-bl cells to monitor viral replication. Luciferase activity was measured at 48 h postinfection. The relative infectivities of the WT and M230L viruses are shown on the y axis, while the x axis denotes the input virus stocks expressed as RT activity. These values translate to an 8-fold virus replication disadvantage for HIV-1M230L compared with the replication capacity of HIV-1WT.