FIG. 6.
RNase H activities of WT and M230L recombinant RTs. (A) Graphic representation of the substrate RNA-DNA (kim40R-kim32D) duplex used to monitor the cleavage efficiency of the M230L and WT RTs. The 40-mer RNA kim40R was labeled at its 5′ terminus with 32P and annealed to the 32-mer DNA oligonucleotide kim32D. (B) RNase H activities were analyzed by monitoring substrate cleavage in time course experiments in the absence (left panel) or the presence (right panel) of a heparin trap. The positions of the cleaved products are indicated on the left side. All reactions were resolved by denaturing 6% polyacrylamide gel electrophoresis.