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Disk diffusion analysis of selected antimicrobials. Disk diffusion assays were performed as previously described (9). Concentrations of amikacin (A), tobramycin (B), and methicillin (C) are given in μg/ml, while the concentration of paraquat (D) is given in μM. The zone of inhibition is defined as the diameter of the zone of clearance surrounding the impregnated filter disk as measured in centimeters. Zones of inhibition were measured after plates were incubated overnight at 37°C.
