TABLE 1.
Chemotactic activities of wild-type hBD1 and hBD3 against neutrophils and monocytes at different NaCl concentrationsa
| hBD | NaCl concn (mM) | % migrating neutrophils | % migrating monocytes |
|---|---|---|---|
| hBD1 | 100 | 13.5 ± 3.4 | 12.5 ± 2.8 |
| 150 | 12.5 ± 1.8 | 24.3 ± 2.8b | |
| 200 | 11.2 ± 2.8 | 10.1 ± 1.4 | |
| hBD3 | 100 | 8.7 ± 2.6 | 7.3 ± 2.1 |
| 150 | 5.4 ± 2.1b | 12.8 ± 3.2b | |
| 200 | 5.0 ± 1.0b | 7.2 ± 1.6 |
a
The peptide concentration was 1.25 μM. The percentage of migrating cells is in reference to the total number of cells in each assay. The data represent the means ± SDs of three replicates.
b
Significantly different (two-tailed unpaired t test, P < 0.05) from the activity of the same molecule against the same cells at 100 mM NaCl.