FIG. 1.

Schematic diagrams of the plasmids constructed in this study. The pAD plasmids are based on integrative plasmid pPL2. They contain four main elements: (i) tandem transcriptional terminators before the promoter to avoid any residual transcription from any upstream promoter, (ii) a constitutive promoter (Phyper) or a promoter of interest (PinlC and PactA), (iii) the hly 5′ UTR sequence, which was described as a stabilizer of the transcript, and (iv) a fluorescence-encoding gene (gfp, yfp, cfp, or cherry).