FIG. 2.

Activity of Pseudoalteromonas sp. 3J6 culture supernatant (SN_3J6) on biofilm formation by Paracoccus sp. 4M6 and Vibrio sp. D01. (A to D) Biofilms of Paracoccus sp. 4M6 (left) and Vibrio sp. D01 (right) were grown in flow cells (FC) for 48 h after a 2-h adhesion step in flow cells without SN_3J6 (A and B) or in the presence of SN_3J6 (C and D). (E to H) Alternatively, bacteria were incubated with SN_3J6 outside the flow cell and washed prior to inoculation (E and F), or the flow cell glass coverslip was coated with SN_3J6 and washed before inoculation of SN_3J6-free bacteria (G and H). Biofilms were stained with Syto 61 red. A 3D shadow projection is shown for each biofilm. Bars, 30 μm.