FIG. 4.
Effect of SN3J6 on biofilm formation by Pseudomonas aeruginosa, Salmonella enterica, and Escherichia coli. Biofilms of P. aeruginosa PAO1YFP (A and D), Salmonella enterica MB1409HcRed (B and E), and Escherichia coli CC118DsRed (C and F) were grown at 20°C for 48 h in LB broth after a 2-h adhesion step without SN3J6 (A, B, and C) or in the presence of SN3J6 (D, E, and F). The yellow (eYFP) or red (HcRed or DsRed) fluorescent protein produced by each strain was detected. A 3D shadow representation and a side-view projection are shown on the top and bottom, respectively, of each panel. For each experiment, maximal thicknesses and biovolumes were calculated from COMSTAT analyses of 12 to 18 image stacks obtained from at least two independent biofilms. Standard deviations were lower than 10% of the values. Bars, 30 μm.