TABLE 1.
Primers used in this studya
| Primer | Sequence (5′→3′) | Position (bp) in gene | Tm (°C)b | Amplicon length (bp) |
|---|---|---|---|---|
| gadT1F | CGCAATAACGGCATCTCTTT | 48-67 | 63.8 | 249 |
| gadT1R | ACCGACTGTGATTTGGAACC | 278-297 | 63.7 | |
| gadD1F | CGCTTCGGATATGAGGGTTA | 988-1007 | 63.6 | 226 |
| gadD1R | AGTGGATACGCCGGTACTTG | 1195-1214 | 63.7 | |
| gadD3F | GAAACGCTCGAGAAAAATGC | 61-80 | 63.6 | 237 |
| gadD3R | AGTTTGGTCGTTTTGCCTGT | 279-298 | 63.3 | |
| gadT2F | CACGGCTAAAATCGCAAAAT | 438-457 | 63.5 | 207 |
| gadT2R | GGAAGCTTCAACACCCATGT | 626-645 | 63.8 | |
| gadD2F | AATACCTTGCCCATGCAGTC | 1049-1068 | 63.7 | 268 |
| gadD2R | GGCTTGGAAATCTTGGATGA | 1298-1317 | 63.8 | |
| 16SF | TGGGGAGCAAACAGGATTAG | 914-933 | 63.8 | 213 |
| 16SR | TAAGGTTCTTCGCGTTGCTT | 1107-1127 | 63.6 |
a
This study was the source of all primers shown.
b
Tm, melting temperature.