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Assay of P. fluorescens GK13 P(3HO) depolymerase activity in polyacrylamide gels containing 0.1% (wt/vol) SDS. A sample (1.5 μg) of the pure enzyme was incubated with the denaturing cocktail containing 2.5% (vol/vol) 2-mercaptoethanol (A and B) or without 2-mercaptoethanol (C and D) and then analyzed by SDS-PAGE (A and D). Finally, the activity of the depolymerase was assayed by layering the polyacrylamide gel onto an agarose plate with P(3HO) latex (B and C).
