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Effect of immobilization on Accurel MP-1000 on thermal stability of pure P. fluorescens GK13 P(3HO) depolymerase. Samples of the soluble (○) or immobilized (•) enzyme were incubated for 15 min at the indicated temperatures and then immediately cooled to 4°C. Finally, the residual activity was determined at 30°C using P(3HO) as substrate.
