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Effects of SDS and sodium and calcium ions on MALDI MS analysis. The mass spectra obtained without EDTA ammonium added to the CMBT matrix solution are shown in the left panels: lipid A samples were spiked with final concentrations of 10 μM SDS (a), 100 μM NaCl (b), and 10 μM CaCl2 (c). The right panels represent the corresponding spectra with the addition of 20 mM EDTA ammonium to CMBT matrix solution. All mass spectra were acquired in the negative-ion detection mode.
