TABLE 1.

Predicted gene products and phylogeny of putative antibiotic resistance determinants from metagenomic clones and plasmid pAS1 based on BLASTx comparison with the GenBank nr/nt database

Sample	Source	Antibiotic resistance	Top BLASTx result	Nearest neighbor	% identity/% similarity
A4	Bacterial genome	Cm	PKD-repeating protein	Cytophaga hutchinsonii	39/54
A5	Bacterial genome	Cm	No significant hits found	N/Aa	N/A
A7	Bacterial genome	Cm	Hypothetical protein FB2170	Flavobacteriales bacterium HTCC2170	59/76
A9	Bacterial genome	Cm	Hypothetical protein sce4763	Sorangium cellulosum	57/75
A10	Bacterial genome	Cm	Large subunit of N,N-dimethylformidase	Hoeflea phototrophica DFL-43	31/48
A12	Bacterial genome	Cm	Class V aminotransferase	Hyphomonas neptunium	33/45
B4	Bacterial genome	Cm	Hypothetical protein Mpe_A2882	Methylibium petroleiphilum	67/77
D10	Bacterial genome	Amp	PBP, Class C β-lactamase	Spirosoma linguale	66/80
E5	Bacterial genome	Km	O-Methyltransferase	Microscilla sp.	55/62
pAS1	Plasmid	Km	Aminoglycoside phosphotransferase	Rhodopseudomonas palustris	70/83
pAS1	Plasmid	Amp	TEM β-lactamase	Bacillus sp. BT-192	95/96
Ph-A8	Phage genome	N/A	Acriflavin resistance protein	Xanthobacter autotrophicus	53/74
Ph-C2	Phage genome	N/A	Drug resistance transporter, Bcr/CflA subfamily	Serratia proteamaculans	96/98
Ph-C3	Phage genome	N/A	Putative transmembrane multidrug efflux system lipoprotein	Ralstonia pickettii	95/97
Ph-D8	Phage genome	N/A	Class A β-lactamase	Capnocytophaga ochracea	98/98
Ph-E10	Phage genome	N/A	TetC protein	Chlamydia suis	89/92
Ph-G10	Phage genome	N/A	Glyoxalase/bleomycin resistance protein	Stenotrophomonas maltophilia	84/89

^aN/A, not applicable.