FIG. 2.
Application of pH indicator for monitoring of sensitive pH differences. Wild-type and ΔscbR cells were grown in media containing N-acetylglucosamine/threonine (NT) and glucose/glycine (GG) without pH indicator (A), with 0.01% (wt/vol) of 4-nitrophenol (B), and with bromothymol blue (C). (D) A linearity curve of bromothymol blue is shown. (E) A630 was measured in glucose/glycine medium supplemented with bromothymol blue from the result shown in panel C. (F) Quantification of pyruvate in glucose/glycine medium using HPLC.